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Japanese B Encephalitis Antibody among 442 race horses in Seoul Race Track was assayed by hemagglutination inhibition test. In the pre-epidemic season of 1980, the serological survey was done on race horses imported from Australia, Japan. Canada and U.S.A. and the positive rate was analysed by age and by imported years. The obtained results were as follows: 1. Among the sera of 442 horses, 162 specimens were H-1 Antibody positive and the rate was 36.6%. 2. Antibody positive rate by year of importation are as follows: 1970~1976 : 80.7% (42/52) 1977 : 60.2% (50/83) 1978 : 32.6% (30/92) 1979 : 23.8% (25/105) 1980 : 13.6% (15/110) 3. Antibody positive rate by year of horses examined are as follow: 2-3 years : 0 4 years : 15.6% (18-115) 5 years : 26.3% (25/95) 6 years : 48.6% (37/76) 7 years : 54.5% (30/55) 8 years : 90.0% (27/30) over 9 years : 96.1% (25/26) 4. The hones imported from Australia have stayed H-1 antibody negative for Japanese B encephalitis for 6 months after the importation.