Figure 1
Case and control definitions by both body mass index (BMI) and plasma adiponectin concentration.
Q, stands for quintile.
Table 1Characteristics of the study population
Variables |
Categories |
Men (n = 711) |
Women (n = 275) |
|
|
Mean±SD
|
Mean±SD
|
Age (yr) |
|
49.5±9.8 |
51.2± 10.4 |
Weight (kg) |
|
71.6±9.5 |
57.6±7.0 |
Height (cm) |
|
169.7±5.9 |
156.4±5.4 |
Body mass index (kg/m2) |
|
24.8± 2.7 |
23.6±2.7 |
Adiponectine (μς/mL) |
|
6.2±3.3 |
9.1 ±4.7 |
Insulin (IU/mL) |
|
3.4± 1.8 |
3.6±2.2 |
|
|
%
|
%
|
Smoking status |
Non-smokers |
18.7 |
92.4 |
|
Ex-smokers |
38.0 |
2.8 |
|
Current smokers |
43.3 |
3.8 |
Alcohol consumption |
Non-drinkers |
31.1 |
81.8 |
|
Any alcohol drinkers |
68.9 |
18.2 |
Table 2Marker information for three SNPs located in the ADIPOQ gene (3q27)
SNP |
Physicalposition |
Location in ADPOQ
|
Alleles*
|
MAP |
HWE (p) |
D´\r2‡
|
1 |
2 |
M1 |
M2 |
M3 |
-11377C>G
|
188042168 |
5'-2 kb |
C |
G |
0.24 |
0.60 |
- |
0.83 |
0.06 |
45T>G
|
188053586 |
exon |
T |
G |
0.28 |
0.24 |
0.08 |
- |
1 |
276G>T
|
188053817 |
intron |
G |
T |
0.28 |
0.61 |
0 |
0.14 |
- |
Table 3Variations in BMI (kg/mg2) and adiponectin concentration (µg/mL) by genotype of the three SNPs
SNP |
Genotype |
n |
BMI (Mean±SE) |
Adiponectin (Mean ± SE) |
-11377C>G
|
CC |
534 |
24.42 ±0.12 |
7.10 ± 0.16 |
|
CG |
373 |
24.43 ±0.14 |
6.89 ±0.19 |
|
GG |
79 |
25.09±0.30 |
6.71 ±0.42 |
|
p-value |
|
0.09 |
0.28 |
45T> G
|
TT |
533 |
24.46 ±0.12 |
7.00 ±0.16 |
|
GT |
372 |
24.52±0.14 |
6.90 ±0.19 |
|
GG |
81 |
24.34 ±0.30 |
7.69 ±0.42 |
|
p-value |
|
0.91 |
0.26 |
276G>T
|
GG |
501 |
24.38 ±0.12 |
6.94 ±0.17 |
|
GT |
395 |
24.57±0.14 |
7.16 ± 0.19 |
|
TT |
90 |
24.58 ±0.28 |
6.70 ±0.40 |
|
p-value |
|
0.52 |
0.53 |
Table 4Risk estimations for alleles and genotypes of individual markers by three levels of BMI and adiponectin concentration
SNP |
|
G1
|
G2
|
G3
|
Case |
Control |
OR (95% CI) |
Case |
Control |
OR (95% CI) |
Case |
Control |
OR (95% CI) |
-11377C>G
|
C |
72 |
109 |
Reference |
180 |
242 |
Reference |
356 |
416 |
Reference |
|
G |
38 |
33 |
1.73 (0.99-3.02) |
82 |
78 |
1.43 (0.99-2.06) |
162 |
128 |
1.48 (1.13-1.94) |
45T> G
|
T |
86 |
100 |
Reference |
204 |
241 |
Reference |
401 |
394 |
Reference |
|
G |
24 |
42 |
0.64 (0.36-1.14) |
58 |
79 |
0.86 (0.58-1.27) |
117 |
150 |
0.78 (0.59-1.03) |
276G>T
|
G |
77 |
111 |
Reference |
179 |
236 |
Reference |
351 |
393 |
Reference |
|
T |
33 |
31 |
1.57 (0.89-2.80) |
83 |
84 |
1.33 (0.92-1.90) |
167 |
151 |
1.23 (0.95-1.61) |
-11377C>G
|
|
|
|
p = 0.13 |
|
|
p = 0.09 |
|
|
p = 0.06 |
|
CC |
27 |
42 |
Reference |
68 |
91 |
Reference |
129 |
161 |
Reference |
|
GC |
18 |
25 |
1.05 (0.48-2.33) |
44 |
60 |
0.99 (0.60-1.64) |
98 |
94 |
1.32 (0.91-1.91) |
|
GG |
10 |
4 |
3.98 (1.12-14.14) |
19 |
9 |
2.90 (1.23-6.83) |
32 |
17 |
2.30 (1.22-4.34) |
45T> G
|
|
|
|
p = 0.03 |
|
|
p = 0.24 |
|
|
p = 0.23 |
|
TT |
32 |
40 |
Reference |
78 |
96 |
Reference |
154 |
147 |
Reference |
|
GT |
22 |
20 |
1.35 (0.62-2.92) |
48 |
49 |
1.20 (0.73-1.97) |
93 |
100 |
0.90 (0.62-1.29) |
|
GG |
1 |
11 |
0.10 (0.01-0.86) |
5 |
15 |
0.40 (0.14-1.16) |
12 |
25 |
0.47 (0.23-0.97) |
276G>T
|
|
|
|
p = 0.18 |
|
|
p = 0.48 |
|
|
p = 0.47 |
|
GG |
30 |
43 |
Reference |
63 |
90 |
Reference |
122 |
144 |
Reference |
|
GT |
17 |
25 |
1.00 (0.46-2.17) |
53 |
56 |
1.36 (0.83-2.23) |
107 |
105 |
1.19 (0.83-1.71) |
|
TT |
8 |
3 |
4.03 (0.97-16.65) |
15 |
14 |
1.61 (0.72-3.58) |
30 |
23 |
1.54 (0.85-2.80) |
Table 5Risk estimation for 2-3 SNP haplotypes showing statistical significance by three levels of BMI and adiponectin concentration*
SNP
|
G1
|
G2
|
G3
|
-11377C>G
|
45T>G
|
276G>T
|
Case |
Control |
OR (95% CI) |
Case |
Control |
OR (95% CI) |
Case |
Control |
OR (95% CI) |
Model 1 |
|
|
|
|
p = 0.09 |
|
|
p = 0.23 |
|
|
p = 0.01 |
C |
G |
|
22 |
42 |
Reference |
55 |
79 |
Reference |
108 |
147 |
Reference |
C |
T |
|
50 |
67 |
1.50 (0.79-2.86) |
125 |
163 |
1.11 (0.73-1.68) |
248 |
269 |
1.24 (0.91-1.67) |
G |
G |
|
2 |
0 |
- |
3 |
0 |
- |
9 |
3 |
4.02 (1.06-15.21) |
G |
T |
|
36 |
33 |
2.13 (1.05-4.32) |
79 |
78 |
1.48 (0.93-2.36) |
153 |
125 |
1.65 (1.17-2.32) |
Model 2 |
|
|
|
|
p = 3.6×10-3
|
|
|
p = 0.04 |
|
|
p = 0.03 |
C |
|
G |
51 |
95 |
Reference |
128 |
193 |
Reference |
256 |
320 |
Reference |
C |
|
T |
21 |
14 |
2.87 (1.34-6.16) |
52 |
49 |
1.62 (1.03-2.55) |
100 |
96 |
1.30 (0.94-1.79) |
G |
|
G |
26 |
16 |
3.00 (1.46-6.15) |
51 |
43 |
1.80 (1.13-2.86) |
95 |
73 |
1.62 (1.15-2.30) |
G |
|
T |
12 |
17 |
1.31 (0.58-2.99) |
31 |
35 |
1.37 (0.80-2.35) |
67 |
55 |
1.52 (1.03-2.25) |
Model 3 |
|
|
|
|
p = 6.6×10-3
|
|
|
p = 0.04 |
|
|
p<0.05 |
C |
G |
G |
31 |
52 |
Reference |
71 |
101 |
Reference |
138 |
184 |
Reference |
C |
G |
T |
6 |
1 |
11.52 (1.31-101.22) |
17 |
8 |
3.11 (1.27-7.62) |
33 |
26 |
1.68 (0.96-2.94) |
C |
T |
G |
20 |
43 |
0.81 (0.40-1.64) |
57 |
92 |
0.88 (0.56-1.37) |
118 |
136 |
1.14 (0.82-1.59) |
C |
T |
T |
15 |
13 |
1.97 (0.82-4.73) |
35 |
41 |
1.22 (0.71-2.11) |
67 |
70 |
1.26 (0.84-1.88) |
G |
G |
G |
9 |
6 |
2.35 (0.75-7.35) |
16 |
13 |
1.70 (0.77-3.77) |
31 |
28 |
1.48 (0.85-2.58) |
G |
G |
T |
0 |
3 |
- |
2 |
6 |
0.44 (0.09-2.26) |
8 |
12 |
0.88 (0.35-2.22) |
G |
T |
G |
17 |
10 |
2.98 (1.19-7.41) |
35 |
30 |
1.69 (0.95-3.01) |
64 |
45 |
1.87 (1.20-2.91) |