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Hepatitis B Virus(t-IBV) infection is one of the major health problems in Korea and HBsAg positive rate was known to be as 6-10% in general population. This study was carried out to determine HBV infection rate of workers who worked in one company and to assess the conversion rate of HBV markers(HBsAg: by RPHA method, Anti-HBs: PHA method) from 1990 to 1992. A total number of study subject was 1,972 male workers who were interviewed during health check up at 1992. Healthy workers were defined those who have none of the past histories of transfusion, hepatitis, abnormal liver function. The results were as follows: 1. HBsAg positive rate among healthy workers in 1990 was 2.7% and it was highest in age group thirties as 3.0% with no statistically significance by age group. 2. HBsAg positive conversion rate was 3.0% and negative conversion rate was 7.7% from 1990 to 1992. 3. The rate of Anti-HBs formation was 32.5% after 3 doeses hepatitis B vaccination.