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1Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran
2Department of Parasitology and Mycology, School of Medicine, Mazandaran University of Medical Science, Sari, Iran
3Student Research Committee, Mazandaran University of Medical Science, Sari, Iran
4Department of Parasitology, School of Medicine, Sari Branch, Islamic Azad University, Sari, Iran
5School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
©2020, Korean Society of Epidemiology
This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
CONFLICT OF INTEREST
The authors have no conflicts of interest to declare for this study.
FUNDING
None.
AUTHOR CONTRIBUTIONS
Conceptualization: AD, MS, SS. Data curation: AD, SS. Formal analysis: SAH. Funding acquisition: None. Methodology: LI, SAH, AA, MTR, TN. Project administration: AD, SS. Writing –original draft: LI, SAH, TN. Writing – review & editing: LI, SS, SAH, AA, MS, MTR, TN, AD.
Parasite | Primer | Size, bp | Annealing temperature, °C | Time, sec | Reference |
---|---|---|---|---|---|
N. caninum | NP21+: 5’-CCCAGTGCGTCCAATCCTGTAAC-3’ | 337 | 63 | 60 | [16] |
NP6+: 5’-CTCGCCAGTCAACCTACGTCTTCT-3’ | |||||
H. heydorni | JS4: 5’-CGAAATGGGAAGTTTTGTGAAC-3’ | 267 | 65 | 60 | [13] |
JS5: 5’-CAGCAGCTAGATACGTAGA-3’ | |||||
T. gondii | TOX4: 5’-CGCTGCAGGGAGGAAGACGAAAGTTG-3’ | 529 | 55 | 30 | [17] |
TOX5: 5’-CGCTGCAGACACAGTGCATCTGGATT-3’ | |||||
H. hammondi | Hham34F: 5’- ATCCCATTCCGGCTTCAGTCTTTC-3’ | 282 | 60 | 60 | [18] |
Hham3R: 5’-ACAGCGGAGCCGAAGTTGGTTTR-3’ | |||||
B. besnoiti | ITS-1F: 5’-TGACATTTAATAACAATCAACCCTT-3’ | 230 | 58 | 90 | [19] |
ITS-1R: 5’-GGTTTGTATTAACCAATCCGTGA-3’ |
Animals | T. gondii | N. caninum | Hammondia spp. | B. besnoiti |
---|---|---|---|---|
Dog | 1/120 (0.8) | 2/120 (1.7) | 0/120 (0.0) | 0/120 (0.0) |
Cat | 18/80 (22.5) | 0/80 (0.0) | 0/80 (0.0) | 0/80 (0.0) |
Parasite | Primer | Size, bp | Annealing temperature, °C | Time, sec | Reference |
---|---|---|---|---|---|
N. caninum | NP21+: 5’-CCCAGTGCGTCCAATCCTGTAAC-3’ | 337 | 63 | 60 | [16] |
NP6+: 5’-CTCGCCAGTCAACCTACGTCTTCT-3’ | |||||
H. heydorni | JS4: 5’-CGAAATGGGAAGTTTTGTGAAC-3’ | 267 | 65 | 60 | [13] |
JS5: 5’-CAGCAGCTAGATACGTAGA-3’ | |||||
T. gondii | TOX4: 5’-CGCTGCAGGGAGGAAGACGAAAGTTG-3’ | 529 | 55 | 30 | [17] |
TOX5: 5’-CGCTGCAGACACAGTGCATCTGGATT-3’ | |||||
H. hammondi | Hham34F: 5’- ATCCCATTCCGGCTTCAGTCTTTC-3’ | 282 | 60 | 60 | [18] |
Hham3R: 5’-ACAGCGGAGCCGAAGTTGGTTTR-3’ | |||||
B. besnoiti | ITS-1F: 5’-TGACATTTAATAACAATCAACCCTT-3’ | 230 | 58 | 90 | [19] |
ITS-1R: 5’-GGTTTGTATTAACCAATCCGTGA-3’ |
Animals | T. gondii | N. caninum | Hammondia spp. | B. besnoiti |
---|---|---|---|---|
Dog | 1/120 (0.8) | 2/120 (1.7) | 0/120 (0.0) | 0/120 (0.0) |
Cat | 18/80 (22.5) | 0/80 (0.0) | 0/80 (0.0) | 0/80 (0.0) |
Values are presented as number (%).