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The epidemiology of amoebiasis in Thi-Qar Province, Iraq (2015-2020): differentiation of Entamoeba histolytica and Entamoeba dispar using nested and real-time polymerase chain reaction
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Mohammed Hassan Flaih, Ruaa Majid Khazaal, Manar Karem Kadhim, Khwam Reissan Hussein, Falah Abd Bashir Alhamadani
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Epidemiol Health. 2021;43:e2021034. Published online May 6, 2021
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DOI: https://doi.org/10.4178/epih.e2021034
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Abstract
OBJECTIVES
The objective of this study was to evaluate the present status of amoebiasis in Thi-Qar Province in southern Iraq, and to determine the presence of <i>Entamoeba histolytica</i> and <i>Entamoeba dispar</i> with nested and real-time polymerase chain reaction (PCR).
METHODS Epidemiological data were obtained from the public health department of the Thi-Qar Health Office (2015-2020). Eighty stool samples were also randomly collected from patients ≤12 year of age with diarrhea at 2 hospitals between the beginning of February 2020 and the end of October 2020. These samples were selected after microscopy to identify the <i>18S rRNA</i> gene in <i>Entamoeba</i> DNA.
RESULTS Of the 341,554 cases of intestinal parasitic infections, 38,004 (11.1%) individuals were recorded as having amoebiasis, which accounted for the highest proportion of infections in 2015 (26.1%) and the lowest in 2020 (8.1%). Amoebiasis was distributed among all age groups, with the age group of 5-14 years accounting for the highest proportion (27.3%). In molecular testing, 42 (52.5%) out of 80 samples were positive for the <i>18S rRNA</i> gene (888 bp). Using nested PCR, <i>E. histolytica</i> (439 bp) was detected in 25 (31.3%) samples and <i>E. dispar</i> (174 bp) in 14 (17.5%), while using real-time PCR, <i>E. histolytica</i> and <i>E. dispar</i> were detected in 28 (35.0%) and 15 (18.8%) samples, respectively.
CONCLUSIONS Epidemiological data confirmed that amoebiasis is endemic in this province, and is not limited to certain months. Our study confirms the applicability of molecular identification to detect pathogenic and non-pathogenic <i>Entamoeba</i> to prescribe the appropriate drug.
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Summary
Key Message
Our study conducted an epidemiologic evaluation of amoebiasis (2015-2020). Amoebiasis is considered a health burden and uncontrolled disease, is endemic in Thi-Qar Province, and is not related with certain months. This study suggests that molecular diagnosis is necessary before the treatment. Both Nested and RT PCR assays are beneficial to diagnose Entamoeba spp.
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Citations
Citations to this article as recorded by  - Molecular Detection of a Pathogenic Entamoeba among Symptomatic Children in Eastern Kurdistan of Iraq
Sham Jamil Abdullah, Shahnaz Abdulkader Ali
Polish Journal of Microbiology.2024; 73(1): 99. CrossRef - Molecular identification of Entamoeba spp. in humans and cattle in Baghdad, Iraq
Sahad M. K. Al-Dabbagh, Haider H. Alseady, Enas J. Alhadad
Veterinary World.2024; : 1348. CrossRef - Food and Drinking Water as Sources of Pathogenic Protozoans: An Update
Franca Rossi, Serena Santonicola, Carmela Amadoro, Lucio Marino, Giampaolo Colavita
Applied Sciences.2024; 14(12): 5339. CrossRef - Global burden and trends of the Entamoeba infection-associated diseases from 1990 to 2019: An observational trend study
Xiaofang Fu, Yonghong Zhong, Liyan Chen, Minjie Ge, Min Yu, Yibo Sun, Linfeng Shen
Acta Tropica.2023; 240: 106866. CrossRef
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