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To evaluate the degree of bacteria and egg contamination of hand in enterobiasis cases in relation with degree of enterobiasis infection, a total of 35 orphans was divided into 3 groups; 10 children who gave 3 consecutively negative anal swab for Enterobias eggs (Group I), 10 children with alternately positive results (Group II) and 15 children with consecutively positive results (Group III). Those children were examined for 4 times during 4 month period, for coliform bacterial contamination by culturing the hand washing water on the Endo broth, and for Enterobias eggs by brine flotation technique. The results were summerized as follows: 1. By 12 times of follow-up anal swab during 4 month period, the mean positive rate was 21.7% in Group I, 48.3% in Group II and 91.0% in Group III respectively. 2. The positive culture rate 100 ml in 1:100 dilution millipore filtration on Endo broth for coliform bacteria in hand washing were 0% in Group I, 15% (10-20%) in Group II and 38.3% (33.3-56.7%) in Group III respectively. 3. The detection of Enterobias eggs in hand washing and under-nail chit was 0% in Group I, 2.5% (0-10%) in Group II and 15% (6.7-20%) in Groups. The mean number of eggs detected in each positive case was 1.2 in Groups II & III. From the above results, it was concluded that the coliform - bacterial and Enterobias egg contamination of hand was in good correlation with the degree of anal swab positive rates, and that digital contamination itself, in turn, may play the role in later Enterobias infection.