KSE
Search
- Page Path
- HOME > Search
Original Article
- An epidemiological comparative study on diagnosis of rodent leptospirosis in Mazandaran Province, northern Iran
- Behzad Esfandiari, Mohammad Reza Pourshafie, Mohammad Mehdi Gouya, Pejvak Khaki, Ehsan Mostafavi, Jamshid Darvish, Soheila Moradi Bidhendi, Hamed Hanifi, Hossein Nahrevanian
- Epidemiol Health. 2015;37:e2015012. Published online February 23, 2015
- DOI: https://doi.org/10.4178/epih/e2015012
- 21,637 View
- 183 Download
- 16 Web of Science
- 12 Crossref
-
Abstract
PDF -
AbstractOBJECTIVES
Leptospirosis is a zoonosis caused by leptospires, in which transmission occurs through contact with contaminated biological fluids from infected animals. Rodents can act as a source of infection for humans and animals. The disease has a global distribution, mainly in humid, tropical and sub-tropical regions. The aim of this study was to compare culture assays, the microscopic agglutination test (MAT), polymerase chain reaction (PCR), and nested PCR (n-PCR), for the diagnosis of leptospirosis in rodents in Mazandaran Province, northern Iran.METHODSOne hundred fifty-one rodents were trapped alive at 10 locations, and their urine and kidney samples were collected and used for the isolation of live Leptospira. The infecting serovars were identified and the antibody titres were measured by MAT, using a panel of 20 strains of live Leptospira species as antigens. The presence of leptospiral DNA was evaluated in urine and kidney samples using PCR and n-PCR.RESULTSNo live leptospires were isolated from the kidney and urine samples of the rodents. Different detection rates of leptospirosis were observed with MAT (21.2%), PCR (11.3%), and n-PCR (3.3%). The dominant strain was Leptospira serjoehardjo (34.4%, p=0.28), although other serotypes were also found. The prevalence of positive leptospirosis tests in rodents was 15.9, 2.6, and 2.6% among Rattus norvegicus, R. rattus, and Apodemus sylvaticus, respectively.CONCLUSIONSLeptospirosis was prevalent in rodents in Mazandaran Province, northern Iran. MAT was able to detect leptospires more frequently than culture or PCR. The kidney was a more suitable site for identifying leptospiral DNA by n-PCR than urine. Culture was not found to be an appropriate technique for clinical diagnosis. -
Summary
Citations
Citations to this article as recorded by
- Clinico Epidemiological Study of Human Leptospirosis in Hilly Area of South India-A Population Based Case Control Study
Sharvanan E. Udayar, Narasimha B. Chengalarayappa, Ashwini Madeshan, Manjunatha Shivanna, Krishnaveni Marella
Indian Journal of Community Medicine.2023; 48(2): 316. CrossRef - Seroepidemiology of leptospirosis in livestock and workers of high-risk occupation in Kurdistan, Iran
Shadi Aghamohammad, Arian Hajijafari Anaraki, Maryam Rahravani, Mojtaba Rastin, Reza Atashin Sadaf, Meysam Moravedji, Mahdi Rohani
Comparative Immunology, Microbiology and Infectious Diseases.2022; 82: 101758. CrossRef - Epidemiology of Leptospirosis: The First Literature Review of the Neglected Disease in the Middle East
Elena Harran, Christo Hilan, Zouheira Djelouadji, Florence Ayral
Tropical Medicine and Infectious Disease.2022; 7(10): 260. CrossRef - Rodent Species Diversity and Occurrence of Leishmania in Northeastern Iran
Seyed Farzad Motevalli-Haghi, Ghorban Mohammad Ozbaki, Nasibeh Hosseini-Vasoukolaei, Seyed Hassan Nikookar, Omid Dehghan, Jamshid Yazdani-Charati, Roohollah Siahsarvie, Reza Dehbandi, Mahmoud Fazeli-Dinan, Ahmadali Enayati
Polish Journal of Ecology.2021;[Epub] CrossRef - Leptospirosis: Increasing importance in developing countries
Zoey May Pheng Soo, Naveed Ahmed Khan, Ruqaiyyah Siddiqui
Acta Tropica.2020; 201: 105183. CrossRef - Impacts of flood on health of Iranian population: Infectious diseases with an emphasis on parasitic infections
Azar Shokri, Sadaf Sabzevari, Seyed Ahmad Hashemi
Parasite Epidemiology and Control.2020; 9: e00144. CrossRef - Diagnosis of Leptospirosis in Febrile Patients of Golestan Province, Iran: Serology versus PCR
Saied Reza Naddaf, Reyhaneh Mohabati, Rouhollah Vahabpor, Sabah Naeimi, Sana Eybpoosh
Journal of Medical Microbiology and Infectious Diseases.2020; 8(3): 98. CrossRef - Rodent-borne and rodent-related diseases in Iran
Vahid Kazemi-Moghaddam, Rouhullah Dehghani, Mostafa Hadei, Samaneh Dehqan, Mohammad Mehdi Sedaghat, Milad Latifi, Shamim Alavi-Moghaddam
Comparative Clinical Pathology.2019; 28(4): 893. CrossRef - Leptospira infection in rats: A literature review of global prevalence and distribution
Kenneth Boey, Kanae Shiokawa, Sreekumari Rajeev, Nicholas P. Day
PLOS Neglected Tropical Diseases.2019; 13(8): e0007499. CrossRef - Rodent-borne diseases and their public health importance in Iran
Mohammad Hasan Rabiee, Ahmad Mahmoudi, Roohollah Siahsarvie, Boris Kryštufek, Ehsan Mostafavi, Peter J. Krause
PLOS Neglected Tropical Diseases.2018; 12(4): e0006256. CrossRef - In memoriam Jamshid Darvish
Christiane Denys, François Bonhomme, Boris Kryštufek, Marjan Mashkour, Jean Denis Vigne, Roohollah Siahsarvie
Mammalia.2018; 82(6): 521. CrossRef - Epidemiological Distribution of Rodents as Potent Reservoirs for Infectious Diseases in the Provinces of Mazandaran, Gilan and Golestan, Northern Iran
Behzad Esfandiari, Hossein Nahrevanian, Mohammad Reza Pourshafie, Mohammad Mehdi Gouya, Pejvak Khaki, Ehsan Mostafavi, Jamshid Darvish, Hamed Hanifi
Infectious Disease Reports.2017; 9(2): 6900. CrossRef
First
Prev
